The OTS-5000 Oscillating Tissue Slicer represents the most thoughtfully designed solution to sectioning either fixed or fresh tissue, and can prepare sections as thin as 5 microns.
Traditionally once removed from the animal, tissue is immediately cooled to close to +4° C to lower the oxygen demand and prevent anoxia.
Once removed from the animal, tissue must be immediately cooled to lower the oxygen demand and thus preventing anoxia. Some workers consider that perfusing brain before removal is preferable.