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TEMPERATURE CONTROLLED AUTOCLAVABLE TISSUE BATH

  • TEMPERATURE CONTROLLED AUTOCLAVABLE TISSUE BATH
  • Once removed from the animal, tissue must be immediately cooled to lower the oxygen demand and thus preventing anoxia. Some workers consider that perfusing brain before removal is preferable. Cardiomyocetes are more robust to low oxygen concentrations but tissue slices better at lower temperatures.

    Holding the tissue close to 4° C must continue throughout the slicing procedure. This can be done with passive cooling where a known amount of ice is used to maintain the cooled a.c.s.f. or with an electronically controlled Tissue Bath Cooler.


    keyword : imaging, slice, tissue bath, campden instruments ltd

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