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Excised Patch Chamber with Two Slotted Open Baths RC-28
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The RC-28 is a special chamber containing two bath compartments separated by an adjustable dam. The dam functions both as a gate and barrier between the baths. The composition and flow rate of each bath can be independently controlled, and each bath has a separate stainless steel suction tube for perfusion.
The dual slotted, open bath design allows for the exchange of solution surrounding an isolated membrane patch without contaminating or modifying the rest of the population. Raising the solution level in the dam area joins the two baths, while lowering the solution level isolates the two baths. After patching onto a cell in the large bath compartment, the dam is flooded, enabling secure transfer of the electrode attached cell to the smaller compartment. The dam is then drained isolating the two baths.
keyword : electrophysiology, patch chamber, slice, tissue bath, warner instruments
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