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Tissue chambers and microscope stage adapters
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The BT-1xxx tissue chambers all have inflow and outflow via a thin slit to promote laminar flow for flow rates up to 2ml/min. Typical flow rates for the BT-1, BT-1AR are 1ml/min.
In applications using an inverted microscope with air type objective and upright microscope you can use a transparent ITO heater (eg. HI-24p, HI-25Dp) to heat the chamber directly from below.
If perfusing, the inflow is heated with the HPRE2 Pre-heater which connects to the inlet tube. The chambers have a thermistor groove to allow permanent placement of the thermistor sensor for measurement/control of the chamber temperature. With upright microscopes you could also heat the objective with an objective heater (eg. HLS-1p)
keyword : electrophysiology, patch chamber, slice, tissue bath, cell microcontrols
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