Tradition epiflourescence requires optical filter cubes to be positioned in a turret inside the microscope. However, filter-switching time is too long to allow for observation of many biological processes. The addition of an Optospin IV filter wheel allows excitation and emission filters to be changed rapidly (approximately 25 milliseconds). This rapid changing of filters improves the time resolution of FRET and ratiometric imaging techniques.
keyword : imaging, filter, wheel, chroma